Aliases
SSB protein, 2.5
Antibody Type
Polyclonal Antibody
Species
Enterobacteria phage T7
Uniprot ID
P03696
Immunogen
Recombinant Enterobacteria phage T7 Single-stranded DNA-binding protein gp2.5 protein (1-232AA)
Raised In
Rabbit
Species Reactivity
Enterobacteria phage T7
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
Single-stranded DNA-binding protein that eliminates secondary structure in long ssDNA formed on the lagging strand of the replication fork. Stimulates DNA polymerase activity and increases the efficiency of RNA primer synthesis by interacting with the DNA polymerase and the helicase/primase protein gp4. Disrupts loops, hairpins and other secondary structures present on ssDNA to reduce and eliminate pausing of viral DNA polymerase at specific sites during elongation.
Isotype
IgG
Conjugate
FITC
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Literature
[1]”Essential residues in the C terminus of the bacteriophage T7 gene 2.5 single-stranded DNA-binding protein.” Marintcheva B., Hamdan S.M., Lee S.J., Richardson C.C. J. Biol. Chem. 281:25831-25840(2006). [2]”A unique loop in T7 DNA polymerase mediates the binding of helicase-primase, DNA binding protein, and processivity factor.” Hamdan S.M., Marintcheva B., Cook T., Lee S.J., Tabor S., Richardson C.C. Proc. Natl. Acad. Sci. U.S.A. 102:5096-5101(2005). [3]”Acidic carboxyl-terminal domain of gene 2.5 protein of bacteriophage T7 is essential for protein-protein interactions.” Kim Y.T., Richardson C.C. J. Biol. Chem. 269:5270-5278(1994).
