Aliases
4EBP1; EIF4EBP1; P/OKCL.6; PHAS-1;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q13541
NCBI Gene ID: 13685
Immunogen
Synthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 70 (A-K-T-P-P).
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Western blotting: 1:500~1:3000, Immunohistochemistry: 1:50~1:100,
Background / Function
Regulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.Anne-Claude Gingras, Genes & Dev., Jun 1999; 13: 1422. Guangming Liu, J. Biol. Chem., Mar 2002; 277: 8810 – 8816. Ya-Ping Jiang, J. Biol. Chem., Mar 2001; 276: 10943 – 10951. Anne-Claude Gingras, Genes & Dev., Feb 1998; 12: 502.
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
