Aliases
4EBP1; EIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Insulin-stimulated EIF-4E binding protein PHAS-I; P/OKCL.6
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q13541
NCBI Gene ID: 1978
Immunogen
Synthesized peptide derived from Internal of human 4E-BP1.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
IHC Recommended dilution: Immunohistochemistry: 1:50~1:100
Background / Function
Regulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.Pause A., Nature 371:762-767(1994).Gevaert K., Nat. Biotechnol. 21:566-569(2003).Haghighat A., EMBO J. 14:5701-5709(1995).
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
