Description
Aliases
Rep, 40.4 kDa protein, Protein AC1, Protein AL1, AC1, AL1
Antibody Type
Polyclonal Antibody
Species
African cassava mosaic virus
Uniprot ID
P14982
Immunogen
Recombinant African cassava mosaic virus Replication-associated protein (1-358AA)
Raised In
Rabbit
Species Reactivity
African cassava mosaic virus
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5′-TAATATTAC-3′ in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5′-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3′-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities (By similarity).
Isotype
IgG
Conjugate
FITC
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Literature
[1]”Nucleotide sequence of cassava latent virus DNA.” Stanley J., Gay M.R. Nature 301:260-262(1982).Additional information
| Size | 50μg, 100μg |
|---|
