Description
Aliases
Type I DHQase, Type I dehydroquinase, DHQ1, STY1760, t1231, aroD
Antibody Type
Polyclonal Antibody
Species
Salmonella typhi
Uniprot ID
P24670
Immunogen
Recombinant Salmonella typhi 3-dehydroquinate dehydratase protein (1-252AA)
Raised In
Rabbit
Species Reactivity
Salmonella typhi
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids. Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate. The reaction involves the formation of an imine intermediate between the keto group of 3-dehydroquinate and the epsylon-amino group of Lys-170 at the active site.
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Modification
3-dehydroquinase
Literature
[1]”Insights into substrate binding and catalysis in bacterial type I dehydroquinase.” Maneiro M., Peon A., Lence E., Otero J.M., Van Raaij M.J., Thompson P., Hawkins A.R., Gonzalez-Bello C. Biochem. J. 462:415-424(2014). [2]”Comparison of different crystal forms of 3-dehydroquinase from Salmonella typhi and its implication for the enzyme activity.” Lee W.H., Perles L.A., Nagem R.A., Shrive A.K., Hawkins A., Sawyer L., Polikarpov I. Acta Crystallogr. D 58:798-804(2002). [3]”The two types of 3-dehydroquinase have distinct structures but catalyze the same overall reaction.” Gourley D.G., Shrive A.K., Polikarpov I., Krell T., Coggins J.R., Hawkins A.R., Isaacs N.W., Sawyer L. Nat. Struct. Biol. 6:521-525(1999).Additional information
| Size | 50μg, 100μg |
|---|
