Aliases
Cholest-4-en-3-one 26-monooxygenase, Cytochrome P450 125, Steroid C27-monooxygenase, cyp125, Rv3545c, MTCY03C7.11
Antibody Type
Polyclonal Antibody
Species
Mycobacterium tuberculosis
Uniprot ID
P9WPP1
Immunogen
Recombinant Mycobacterium tuberculosis Steroid C26-monooxygenase protein (1-433AA)
Raised In
Rabbit
Species Reactivity
Mycobacterium tuberculosis
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
Catalyzes the C-27 hydroxylation of cholest-4-en-3-one and cholesterol and subsequently oxidizes the alcohol of the former to the cholest-4-en-3-one-27-oic acid via the aldehyde intermediate. Not required to incorporate the cholesterol side-chain carbon atoms into cellular lipids.
Isotype
IgG
Conjugate
Biotin
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Modification
Steroid C26-monooxygenase
Literature
[1]”Reverse type I inhibitor of Mycobacteriumtuberculosis CYP125A1.” Ouellet H., Kells P.M., Ortiz de Montellano P.R., Podust L.M. Bioorg. Med. Chem. Lett. 21:332-337(2011). [2]”Mycobacterium tuberculosis CYP125A1, a steroid C27 monooxygenase that detoxifies intracellularly generated cholest-4-en-3-one.” Ouellet H., Guan S., Johnston J.B., Chow E.D., Kells P.M., Burlingame A.L., Cox J.S., Podust L.M., de Montellano P.R. Mol. Microbiol. 77:730-742(2010). [3]”The Structure of Mycobacterium tuberculosis CYP125: molecular basis for cholesterol binding in a P450 needed for host infection.” McLean K.J., Lafite P., Levy C., Cheesman M.R., Mast N., Pikuleva I.A., Leys D., Munro A.W. J. Biol. Chem. 284:35524-35533(2009).
