Description
Aliases
Protein B1, Ribonucleoside-diphosphate reductase 1 R1 subunit, Ribonucleotide reductase 1, b2234, JW2228, dnaF, nrdA
Antibody Type
Polyclonal Antibody
Species
Escherichia coli
Uniprot ID
P00452
Immunogen
Recombinant Escherichia coli Ribonucleoside-diphosphate reductase 1 subunit alpha protein (1-761AA)
Raised In
Rabbit
Species Reactivity
Escherichia coli
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1 contains the binding sites for both substrates and allosteric effectors and carries out the actual reduction of the ribonucleotide. It also provides redox-active cysteines.
Isotype
IgG
Conjugate
Biotin
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Modification
Ribonucleoside-diphosphate reductase 1 subunit alpha
Literature
[1]”Binding specificity and mechanistic insight into glutaredoxin-catalyzed protein disulfide reduction.” Berardi M.J., Bushweller J.H. J. Mol. Biol. 292:151-161(1999). [2]”Binding of allosteric effectors to ribonucleotide reductase protein R1: reduction of active-site cysteines promotes substrate binding.” Eriksson M., Uhlin U., Ramaswamy S., Ekberg M., Regnstroem K., Sjoeberg B.-M., Eklund H. Structure 5:1077-1092(1997). [3]”A new mechanism-based radical intermediate in a mutant R1 protein affecting the catalytically essential Glu441 in Escherichia coli ribonucleotide reductase.” Persson A.L., Eriksson M., Katterle B., Potsch S., Sahlin M., Sjoberg B.M. J. Biol. Chem. 272:31533-31541(1997).Additional information
| Size | 50μg, 100μg |
|---|
