Description
Aliases
Nucleocapsid protein, Protein N, NP
Antibody Type
Polyclonal Antibody
Species
Influenza A virus
Uniprot ID
P03466
Immunogen
Recombinant Influenza A virus Nucleoprotein (1-498AA)
Raised In
Rabbit
Species Reactivity
Influenza A virus
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein’s nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein’s nuclear localization signals, targeting the RNP to the nucleus.
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Research
Others
Literature
[1]”Interaction of the influenza virus nucleoprotein with the cellular CRM1-mediated nuclear export pathway.” Elton D., Simpson-Holley M., Archer K., Medcalf L., Hallam R., McCauley J., Digard P.J. Virol. 75:408-419(2001). [2]”Oligomerization of the infAdditional information
| Size | 50μg, 100μg |
|---|
