Aliases
Adenine-specific methyltransferase EcoRV, M.EcoRV, ecoRVM
Antibody Type
Polyclonal Antibody
Species
Escherichia coli
Uniprot ID
P04393
Immunogen
Recombinant Escherichia coli Modification methylase EcoRV protein (1-298AA)
Raised In
Rabbit
Species Reactivity
Escherichia coli
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
This methylase recognizes the double-stranded sequence GATATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the EcoRV endonuclease.
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Modification
Modification methylase EcoRV
Literature
[1]”Characterization of the genes coding for the Eco RV restriction and modification system of Escherichia coli.” Bougueleret L., Schwarzstein M., Tsugita A., Zabeau M. Nucleic Acids Res. 12:3659-3676(1984). [2]”The EcoRV restriction-modification system: genes, enzymes, synthetic substrates.” Kraev A.S., Kravets A.N., Chernov B.K., Skryabin K.G., Baev A.A. Mol. Biol. (Mosk.) 19:236-242(1985).
