Description
Aliases
Cytosine-specific methyltransferase SacI, sacIM
Antibody Type
Polyclonal Antibody
Species
Streptomyces achromogenes
Uniprot ID
O31073
Immunogen
Recombinant Streptomyces achromogenes Modification methylase SacI protein (1-390AA)
Raised In
Rabbit
Species Reactivity
Streptomyces achromogenes
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
This methylase recognizes the double-stranded sequence GAGCTC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the SacI endonuclease.
Isotype
IgG
Conjugate
Biotin
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Modification
M.SacI
Literature
[1]”Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli.” Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II Mol. Gen. Genet. 260:226-231(1998).Additional information
Size | 50μg, 100μg |
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