Aliases
Glucokinase regulator, GCKR
Antibody Type
Polyclonal Antibody
Species
Human
Uniprot ID
Q14397
Immunogen
Recombinant human Glucokinase regulatory protein (1-625AA)
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
Inhibits glucokinase (GCK) by forming an inactive complex with this enzyme. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity.
Isotype
IgG
Conjugate
FITC
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Literature
[1]Molecular basis for the role of glucokinase regulatory protein as the allosteric switch for glucokinase. Choi J.M., Seo M.H., Kyeong H.H., Kim E., Kim H.S. Proc. Natl. Acad. Sci. U.S.A. 110:10171-10176(2013). [2]The P446L variant in GCKR associated with fasting plasma glucose and triglyceride levels exerts its effect through increased glucokinase activity in liver. Beer N.L., Tribble N.D., McCulloch L.J., Roos C., Johnson P.R., Orho-Melander M., Gloyn A.L. Hum. Mol. Genet. 18:4081-4088(2009). [3]Crystal structure of glucokinase regulatory protein. Pautsch A., Stadler N., Lohle A., Rist W., Berg A., Glocker L., Nar H., Reinert D., Lenter M., Heckel A., Schnapp G., Kauschke S.G. Biochemistry 52:3523-3531(2013)
