Description
Aliases
Postreplication repair protein RAD18, RING finger protein 73, RAD18, RNF73
Antibody Type
Polyclonal Antibody
Species
Human
Uniprot ID
Q9NS91
Immunogen
Recombinant human E3 ubiquitin-protein ligase RAD18 protein (1-495AA)
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on Lys-164. Has ssDNA binding activity.
Isotype
IgG
Conjugate
FITC
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Literature
[1]”Identification of human RAD18 (hHR18), a homolog of yeast RAD18.” Jang Y., Chae S. Submitted (JUL-2000). [2]”A quantitative atlas of mitotic phosphorylation.” Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). [3]”Symmetry and asymmetry of the RING-RING dimer of Rad18.” Huang A., Hibbert R.G., de Jong R.N., Das D., Sixma T.K., Boelens R. J. Mol. Biol. 410:424-435(2011).Additional information
| Size | 50μg, 100μg |
|---|
