CNOT8 Antibody

$138.92$232.63

SKU: 58083 Categories: ,

Description

Aliases

CAF1-like protein, CALIFp, CAF2, CCR4-associated factor 8, Caf1b, CNOT8, CALIF, POP2

Antibody Type

Polyclonal Antibody

Species

Human

Uniprot ID

Q9UFF9

Immunogen

Recombinant human CCR4-NOT transcription complex subunit 8 protein (1-292AA)

Raised In

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, IHC;Recommended dilution:IHC:1:20-1:200

Background / Function

Has 3′-5′ poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT7. Catalytic component of the CCR4-NOT complex which is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.

Isotype

IgG

Conjugate

Unconjugated

Storage Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antigen Affinity Purified

Literature

[1]The anti-proliferative activity of BTG/TOB proteins is mediated via the Caf1a (CNOT7) and Caf1b (CNOT8) deadenylase subunits of the Ccr4-not complex.” Doidge R., Mittal S., Aslam A., Winkler G.S. PLoS ONE 7:E51331-E51331(2012). [2]Initial characterization of the human central proteome.”Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011). [3]CCR4-NOT deadenylates mRNA associated with complexes in human cells.”Piao X., Zhang X., Wu L., Belasco J.G. Mol. Cell. Biol. 30:1486-1494(2010).

Additional information

Size

50μl, 100μl

Certificate of Analysis