Description
Aliases
SMC protein 1A, SMC-1-alpha, SMC-1A, Sb1.8, SMC1A, DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1
Antibody Type
Polyclonal Antibody
Species
Human
Uniprot ID
Q14683
Immunogen
Recombinant human Structural maintenance of chromosomes protein 1A protein (1-130AA)
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
ELISA, IHC;Recommended dilution:IHC:1:20-1:200
Background / Function
Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antigen Affinity Purified
Literature
[1]Hypertrophic cardiomyopathy in a girl with Cornelia de Lange syndrome due to mutation in SMC1A.” Limongelli G., Russo S., Digilio M.C., Masciadri M., Pacileo G., Fratta F., Martone F., Maddaloni V., D’Alessandro R., Calabro P., Russo M.G., Calabro R., Larizza L.Am. J. Med. Genet. A 152:2127-2129(2010). “SMC1A expression and mechanism of pathogenicity in probands with X-Linked Cornelia de Lange syndrome.” Liu J., Feldman R., Zhang Z., Deardorff M.A., Haverfield E.V., Kaur M., Li J.R., Clark D., Kline A.D., Waggoner D.J., Das S., Jackson L.G., Krantz I.D. Hum. Mutat. 30:1535-1542(2009). [2]SMC1A expression and mechanism of pathogenicity in probands with X-Linked Cornelia de Lange syndrome.” Liu J., Feldman R., Zhang Z., Deardorff M.A., Haverfield E.V., Kaur M., Li J.R., Clark D., Kline A.D., Waggoner D.J., Das S., Jackson L.G., Krantz I.D.Hum. Mutat. 30:1535-1542(2009). [3]Cornelia de Lange syndrome mutations in SMC1A or SMC3 affect binding to DNA.” Revenkova E., Focarelli M.L., Susani L., Paulis M., Bassi M.T., Mannini L., Frattini A., Delia D., Krantz I., Vezzoni P., Jessberger R., Musio A.Hum. Mol. Genet. 18:418-427(2009).Additional information
| Size | 50μl, 100μl |
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