Description
Aliases
p105MCM, MCM6
Antibody Type
Polyclonal Antibody
Species
Human
Uniprot ID
Q14566
Immunogen
Recombinant human DNA replication licensing factor MCM6 protein (562-821AA)
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
ELISA, WB, IHC ;Recommended dilution:WB:1:500-2000, IHC:1:20-1:200
Background / Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for ‘once per cell cycle’ DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antigen Affinity Purified
Literature
[1]Characterization and structure determination of the Cdt1 binding domain of human minichromosome maintenance (Mcm) 6.”Wei Z., Liu C., Wu X., Xu N., Zhou B., Liang C., Zhu G.J. Biol. Chem. 285:12469-12473(2010). [2]N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.” Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012). [3]Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features.”Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).Additional information
| Size | 50μl, 100μl |
|---|
