Aliases
FACT complex subunit SPT16, Chromatin-specific transcription elongation factor 140 kDa subunit, FACT 140 kDa subunit, FACTp140, Facilitates chroma, SUPT16H, FACT140, FACTP140
Antibody Type
Polyclonal
Species
Human
Uniprot ID
Q9Y5B9
Immunogen
Recombinant human FACT complex subunit SPT16 protein (187-282AA)
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
ELISA, IHC;Not yet tested in other applications.
Background / Function
Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of ‘Ser-392’ of p53/TP53 via its association with CK2 (casein kinase II).
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
>95%,Protein G purified
Modification
FACT complex subunit SPT16
