Description
Part of the ORF1 a/b gene sequence, Gene E and Gene N coding region sequence of 2019 Novel Coronavirus (2019-nCoV) were obtained by chemical synthesis, and cloned into a retrovirus vector. The pseudovirus was prepared in 293T cells, and then concentrated and purified by ultra-fast centrifugation. The pseudovirus is composed of retrovirus envelope and above sequences. It can be used as a positive control for viral RNA extraction experiment and qPCR test.