Goat a-Rat IgG, Horseradish peroxidase (HRP) labeled (1.0mg/ml)

$308.70

Description

Product name: Goat Anti-Rat IgG H&L (HRP)

Host species: Goat

Target species: Rat

Specificity: By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG and with light chains common to other Rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

Tested applications: Suitable for: Western Blot, ELISA ICC, IHC-P

Conjugation: HRP

Form: Liquid

Storage instructions: Shipped at 4°C. Store at +4°C

Storage buffer: 0.2% BSA, PBS

Preservative: 0.1% Proclin

Concentration: 1 mg/ml 

Purity: Immunogen affinity purified: isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

Conjugation notes: Molar enzyme/ antibody protein ratio is 4:1

Clonality: Polyclonal

Isotype: IgG


Recommended Dilutions: Below are the recommended starting dilutions (optimal dilutions/concentrations) and should be determined by the end user:

Application

Recommended Dilution

Western Blot

1:10000 – 1:50000
Chemiluminescent detection

1:5000 – 1:30000
Colorimetric detection

ELISA

1:10000 – 1:100000

Western Blot – Probed with Goat α-Rat IgG, Horseradish peroxidase (HRP) labeled
All lanes: Anti-Tubulin antibody – Loading Control at 1 µg/ml
Lanes 1-6:  HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1 – 2: Goat α-Rat IgG, Horseradish peroxidase (HRP) at 1:2000 dilution
Lanes 3 – 4: Goat α-Rat IgG, Horseradish peroxidase (HRP) at 1:10000 dilution
Lanes 5 – 6: Goat α-Rat IgG, Horseradish peroxidase (HRP) at 1:20000 dilution
Developed using the ECL technique.

Additional information

Size

1.0ml

Certificate of Analysis