Caspase-5 Substrate (Ac-WEHD-AFC) [1mM], Fluorometric

Description

Caspase-5 Substrate (Ac-WEHD-AFC) [1mM], Fluorometric

General description

This product is a ready-to-use fluorometric substrate for Caspase-1, -4, -5 and related caspases that recognize the amino acid sequence WEHD. Caspase-5 and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate WEHD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.

Synonyms:                        Ac-WEHD-AFC, Caspase-5 Substrate, Fluorogenic

Peptide Sequence:          Ac-Trp-Glu-His-Asp-AFC

Appearance:                     Liquid

Formulation:                    DMSO (1 mM)

Molecular Formula:        C₃₈H₃₇F₃N₈O₁₁

Molecular Weight:           838.8

InChi Key:                         NEGOLAGPKZGIKJ-UHFFFAOYSA-N

PubChem CID:                 44135104

Handling:                          Protect from light and moisture

Storage temp.:                 -20°C

Additional information