Aliases
SLIT-ROBO Rho GTPase activating protein 1
Antibody Type
Polyclonal Antibody
Uniprot ID
Q7Z6B7
Immunogen
Synthetic peptide of human SRGAP1
Raised In
Rabbit
Species Reactivity
Human, Mouse
Tested Applications
ELISA: 1:2000-1:5000; WB:1:500-1:2000; IHC: 1:25-1:100
Background / Function
SRGAP1 contains an FCH (Fes/CIP4 homology) domain, a Rho-GAP domain and an SH3 domain. In the presence of Slit, SRGAP1 (via its SH3 domain) binds to the CC3 motif in robo (a protein responsible for mediating the repulsive effect of Slit) with higher affinity and inhibits Cdc42 activity in a robo/SRGAP-dependent manner. More specifically, SRGAP1 increases the intrinsic GTPase activity of Cdc42, thereby converting it to its inactive, GDP-bound form. Inactivation of Cdc42 ultimately leads to a decrease in actin polymerization.
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antigen affinity purification
