Aliases
Gripap; Grasp; GRIP1-associated protein
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q9JHZ4
NCBI Protein: NP_064522.3
Immunogen
Peptide sequence around aa. 814-818(Q-E-I-V-R ) derived from Rat GRASP.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB Recommended dilution: Predicted MW: 95kd, Western blotting: 1:500~1:1000,
Background / Function
GRASP (GRP1-associated scaffold protein, tamalin) is a 395 amino acid proteinencoded by the human gene GRASP . GRASP is a scaffold protein that com-prises multiple protein-interacting domains, including a 95 kDa postsynaptic density protein (PSD-95)/discs-large/ZO-1 (PDZ) domain, a leucine-zipperregion and a carboxyl-terminal PDZ-binding motif. GRASP is involved with intracellular trafficking and contributes to the macromolecular organization of group 1 metabotropic glutamate receptors (mGluRs) at synapses. GRASP forms a heteromer composed of GRASP , PSCD2 and at least one mGluR-1. It also interacts with PSCD3, mGluR-2, mGluR-3 and mGluR-5. GRASP is highly expressed in brain and has lower levels of expression in lung, heart, embryo, kidney and ovary.Kitano, J., Kimura, K., Yamazaki, Y., Soda, T., Shigemoto, R., Nakajima, Y.and Nakanishi, S. 2002. Tamalin, J. Neurosci. 22: 280-1289.Hall, B.S., Gabernet-Castello, C., Voak, A., Goulding, D., Natesan, S.K. and Field, M.C. 2006. J. Biol. Chem. 281: 27600-27612. Sugi, T., Oyama, T., Muto, T., Nakanishi, S., Morikawa, K. and Jingami, H. 2007. Crystal structures of autoinhibitory PDZ domain of Tamalin EMBO J.26: 2192-2205.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide
