Aliases
ASH2; Bre2; ASH2L1; ASH2L2;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q9UBL3
NCBI Protein: NP_004665.2
Immunogen
Peptide sequence around aa.620~624(G-R-R-S-P) derived from Human ASH2L.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB Recommended dilution: Predicted MW: 65 80kd, Western blotting: 1:500~1:1000,
Background / Function
Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates ‘Lys-4’ of histone H3, but not if the neighboring ‘Lys-9’ residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at ‘Lys-4’ of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis. Wysocka J., Myers M.P., Laherty C.D., Eisenman R.N., Herr W.Genes Dev. 17:896-911(2003)Patel A., Dharmarajan V., Vought V.E., Cosgrove M.S.J. Biol. Chem. 284:24242-24256(2009)
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
