Aliases
Piwi-like RNA-mediated gene silencing 4, HIWI2; MIWI2
Antibody Type
Polyclonal Antibody
Uniprot ID
Genbank No.: BC031060
Immunogen
Fusion protein corresponding to a region derived from 546-838 amino acids of human piwi-like RNA-mediated gene silencing 4
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
ELISA WB Recommended dilution: Predicted MW: 97kd , ELISA: 1:2000-1:5000, Western blotting: 1:500-1:2000
Background / Function
PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells. Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.1mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
