Aliases
HLF; MOP2; ECYT4; HIF2A; PASD2
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q99814; NCBI Gene ID: 2034; NCBI mRNA:
Immunogen
Peptide sequence around aa.547-551(P-E-E-R-L) derived from HumanHIF2A.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB Recommended dilution: Predicted MW: 120kd, Western blotting : 1:500~1:1000
Background / Function
Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5′-[AG]CGTG-3′ within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
