Aliases
Thyroid hormone receptor-associated protein complex 230 kDa component; Trap230; Activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q93074
NCBI Gene ID: 9968
Immunogen
Synthesized peptide derived from internal of human MED12.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
IHC Recommended dilution: Immunohistochemistry: 1:50~1:100
Background / Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.Ito M., Mol. Cell 3:361-370(1999).Nagase T., DNA Res. 3:17-24(1996).Philibert R.A., Mol. Psychiatry 3:303-309(1998).
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
