Aliases
ARK5
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: O60285
NCBI Gene ID: 9891
Gen
Immunogen
Synthetic peptide corresponding to a region derived from internal residues of human NUAK family, SNF1-like kinase, 1
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
IHC Recommended dilution: Immunohistochemistry: 1:25-1:100
Background / Function
NUAK family SNF1-like kinase 1 also known as AMPK-related protein kinase 5 (ARK5) is an enzyme that in humans is encoded by the NUAK1 gene. Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of ‘Ser-464’ of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1.
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antigen Affinity Purified
