Nitrocellulose Membrane Precut

Description

General Description

A widely used membrane for different blotting applications that has high affinity for protein binding, supplied pre-cut in 8.5cm x 7.5cm and 10cm x 10cm sizes with blotting paper to make it ready to use. It also demonstrates a high level of compatibility with different detection methods, such as chemiluminescent and chromogenic, etc.

Nitrocellulose membranes are a popular matrix commonly incorporated in protein blotting techniques, employing their high protein-binding affinity, compatibility with various detection methods (chemiluminescence, chromogenic, and fluorescence), and the membrane’s ability to immobilize proteins, glycoproteins, or nucleic acids. Protein immobilization is believed to be a result of hydrophobic interactions. During the process of electrophoretic transfer, high salt and low methanol concentrations help improve protein immobilization to the membrane, especially for higher molecular weight proteins.

Additional information