Aliases
70 kDa zeta-associated protein; SRK; Syk-related tyrosine kinase; ZA70; ZAP-70
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P43403
NCBI Protein: NP _001070.2
Immunogen
Peptide sequence around aa.491~495 (S-Y-Y-T-A) derived from Human ZAP70.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Predicted MW: 70kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the zeta chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-zeta chain, which can mediate the transduction of extracellular stimuli into cellular effector functionsChan AC, et al. (1995) EMBO J; 14(11): 2499-2508Kong G, et al. (1996) Mol Cell Biol; 16(9): 5026-5035Salomon AR, et al. (2003)Proc Natl Acad Sci U S A; 100(2): 443-448
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
