Aliases
SYT1/2; SytI/II; Synaptotagmin I/II; P65; SYT
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P21579/Q8N9I0
NCBI Protein:NP_0011
Immunogen
Peptide sequence around aa.200~204/197~201 (R-K-T-L-N) derived from Human Synaptotagmin 1/2.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IF Recommended dilution: Predicted MW: 46kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background / Function
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse Gustavsson N, et al. Proc Natl Acad Sci U S A. 2008 Mar 11; 105(10):3992-7. Cnops L, et al. Cereb Cortex. 2008 May; 18(5):1221-31. Lynch KL, et al. Mol Biol Cell. 2007 Dec; 18(12):4957-68.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
