Aliases
Apoptosis regulator BAX; membrane isoform alpha; BAXA; Bcl2-associated X protein;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q07812
NCBI Gene ID: 581
Immunogen
Synthesized non-phosphopeptide derived from human Bax around the phosphorylation site of threonine 167 (F-G-T(p)-P-T).
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
WB IF Recommended dilution: Western blotting: 1:500~1:3000Immunofluorescence: 1:100~1:500
Background / Function
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.Oltvai Z.N., Cell 74:609-619(1993).Cuddeback S.M., J. Biol. Chem. 276:20559-20565(2001).Guo B., Nature 423:456-461(2003)
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
