Aliases
hKSR2; Kinase suppressor of Ras 2; KSR2;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q6VAB6
NCBI Gene ID: 283455
Immunogen
Synthesized peptide derived from internal of human KSR2.
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
WB IF Recommended dilution: Western blotting: 1:500~1:3000Immunofluorescence: 1:100~1:500
Background / Function
Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.Ota T., Nat. Genet. 36:40-45(2004).Scherer S.E., Nature 440:346-351(2006).Channavajhala P.L., J. Biol. Chem. 278:47089-47097(2003).
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
