Aliases
5-hydroxytryptamine 2C receptor; 5H2C; 5HT-1C; 5HT1C; 5HT2C
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P28335
NCBI Gene ID: 3358
Immunogen
Synthesized peptide derived from internal of human 5-HT-2C.
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
IHC IF Recommended dilution: Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:500
Background / Function
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca2+ ions from intracellular stores. Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelacortin neurons and the release of CRH that then regulates the release of corticosterone. Plays a role in the regulation of appetite and eating behavior, responses to anxiogenic stimuli and stress. Plays a role in insulin sensitivity and glucose homeostasis.Saltzman A.G., Biochem. Biophys. Res. Commun. 181:1469-1478(1991).Stam N.J., Eur. J. Pharmacol. 269:339-348(1994).Xie E., Genomics 35:551-561(1996).
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
