Aliases
Nuclear mitotic apparatus protein 1; NuMA protein; SP-H antigen;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q14980
NCBI Gene ID: 4926
Immunogen
Synthesized peptide derived from internal of human NUMA1.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB IHC Recommended dilution: Western blotting: 1:500~1:3000, Immunohistochemistry: 1:50~1:100,
Background / Function
Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority if the nuclear volume. Required for maintenance and establishment of the mitotic spindle poles, functionning as a tether linking bulk microtubules of the spindle to centrosomes. May be involved in coordination of the alignment of the mitotic spindle to the cellular polarity axis, which is a prerequisite for asymmetric cell divisions.Yang C.H., J. Cell Biol. 116:1303-1317(1992).Compton D.A., J. Cell Biol. 116:1395-1408(1992).Maekawa T., J. Cell Sci. 105:589-600(1993).
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
