Aliases
ESP1; SEPA
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: Q14674
NCBI Gene ID: 9700
Gen
Immunogen
Synthetic peptide corresponding to a region derived from internal residues of human extra spindle pole bodies homolog 1 (S. cerevisiae)
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Western blotting: 1:200-1:1000, Immunohistochemistry: 1:25-1:100
Background / Function
Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 or STAG2 in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 by ESPL1, or separase, which initiates the final separation of sister chromatids.
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antigen Affinity Purified
