Aliases
EC 2.7.11.1; Gamma-PAK; P21-activated kinase 2; PAK 2; PAK-2
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q13153/Q13177/O75914
NCBI Gene ID:
Immunogen
Synthesized non-phosphopeptide derived from human PAK1/2/3 around the phosphorylation site of threonine 423/402/421 (R-S-T(p)-M-V).
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Western blotting: 1:500~1:3000, Immunohistochemistry: 1:50~1:100,
Background / Function
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.Bernadett Boda, J. Neurosci., Dec 2004; 24: 10816 – 10825. Donna L. McPhie, J. Neurosci., Jul 2003; 23: 6914. Jin-Hun Jung, J. Biol. Chem., Dec 2005; 280: 40025 – 40031. Guo-Lei Zhou, Mol. Cell. Biol., Nov 2003; 23: 8058 – 8069.
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
