Aliases
PIM-1; EC 2.7.11.1;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: P05771;
NCBI Gene#: 5579;
N
Immunogen
Peptide sequence around phosphorylation site of serine 661 (G-F-S(p)-Y-T) derived from Human PKCB.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB Recommended dilution: Western blotting: 1:500~1:1000
Background / Function
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1.Zhu M, Chen J, Jiang H, Miao C (2013) Cardiovasc Diabetol 12, 13 Leonard TA, et al. (2011) Cell 144, 55-66 Qiao M, et al. (2010) Mol Cell 38, 512-23
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser661
