Description
Aliases
WEE1A; WEE1hu; kinase WEE1;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: P30291;
NCBI Gene#: 7465;
N
Immunogen
Peptide sequence around phosphorylation site of Serine 642(S-V-S(p)-L-T) derived from Human WEE1.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB Recommended dilution: Western blotting: 1:500~1:1000
Background / Function
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on ‘Tyr-15’. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on ‘Tyr-15’ and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.Watanabe N., EMBO J. 14:1878-1891(1995).Cichutek A., Cytogenet. Cell Genet. 93:277-283(2001).Igarashi M., Nature 353:80-83(1991)
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser642
Additional information
| Size | 100?l |
|---|
