Aliases
CDC25HU2; CDC25M2; Dual specificity phosphatase Cdc25B; EC 3.1.3.48; M-phase inducer phosphatase 2; MPIP2
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#:P30305;
NCBI Gene#:994
Immunogen
Peptide sequence around phosphorylation site of serine 323 (S-P-S(p)-M-P) derived from Human CDC25B.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB Recommended dilution: Western blotting: 1:500~1:3000,
Background / Function
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser323
