ATR (Phospho-Ser428) Antibody

$500.54

SKU: 38713 Categories: ,

Description

Aliases

ataxia telangiectasia and Rad3-related protein; EC 2.7.11.1; FRAP-related protein; FRP1; kinase ATR; protein kinase ATR

Antibody Type

Polyclonal Antibody

Uniprot ID

Swiss-Prot#:Q13535;
NCBI Gene#:545

Immunogen

Peptide sequence around phosphorylation site of serine 428 (G-I-S(p)-P-K) derived from Human ATR.

Raised In

Rabbit

Species Reactivity

Human

Tested Applications

IHC Recommended dilution: Immunohistochemistry: 1:50~1:100

Background / Function

Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates ‘Ser-139’ of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.

Conjugate

Unconjugated

Storage Buffer

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

liquid

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Modification

Phospho-Ser428

Additional information

Size

100?l

Certificate of Analysis