Description
Aliases
CDC25B
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q9DBN8
NCBI Protein: NP_001104545.
Immunogen
Peptide sequence around phosphorylation site of Serine 149 (F-R-S(p)-L-P) derived from Mouse Cdc25B.
Raised In
Rabbit
Species Reactivity
Mouse Rat
Tested Applications
WB Recommended dilution: Predicted MW: 62kd, Western blotting: 1:500~1:1000
Background / Function
Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. Li G, et al. (2010) Retrovirology.7:59. Wang Z,et al. (2010) BMC Cancer.10:233. Lucci MA,et al. (2010) Cell Oncol.32(5-6):361-72.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Modification
Phospho-Ser149
Additional information
| Size | 100μl |
|---|
