Description
Aliases
ADRB2; PAK 1; P65-PAK; P68-PAK;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: Q13153;
NCBI Gene#: 5058;
N
Immunogen
Peptide sequence around phosphorylation site of Serine 199(T-K-S(p)-V-I) derived from Human PAK1.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.Brown J.L., Curr. Biol. 6:598-605(1996).Sells M.A., Curr. Biol. 7:202-210(1997).The MGC Project Team; Genome Res. 14:2121-2127(2004).
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser199
Additional information
| Size | 100?l |
|---|
