Aliases
P531; TP53B; TP53BP1; TRP53BP1; Tumor suppressor p53-binding protein 1; p53-binding protein 1
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#:Q12888;
NCBI Gene#:7158
Immunogen
Peptide sequence around phosphorylation site of serine 25 (E-D-S(p)-Q-P) derived from Human 53BP1.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
IHC IF Recommended dilution: Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:500
Background / Function
Plays a key role in the response to DNA damage. May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation.
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser25
