Description
Aliases
Eukaryotic translation initiation factor 2 subunit alpha; EIF-2A;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P05198
NCBI Protein: NP_004085.1
Immunogen
Peptide sequence around phosphorylation site of serine 51 (E-L-S(p)-R-R) derived from Human eIF2a.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 38kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Xavier Saelens, et al. (2001) J. Biol. Chem; 276: 41620 – 41628. Hiroyuki Kubota, et al. (2003) J. Biol. Chem ; 278: 20457 – 20460. Shijian Chu, et al. (2006) Am J Physiol Lung Cell Mol Physiol ; 291: L983 – L992. Eileen Connolly, et al. (2006) Mol. Cell. Biol ; 26: 3955 – 3965.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser51
Additional information
| Size | 100?l |
|---|
