Description
Aliases
Efnb3
Antibody Type
Polyclonal Antibody
Species
Mouse
Uniprot ID
O35393
Immunogen
Recombinant mouse Ephrin-B3 protein (28-227AA)
Raised In
Rabbit
Species Reactivity
Mouse
Tested Applications
ELISA, WB;Not yet tested in other applications.
Background / Function
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons.
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Literature
[1]Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins. Wollscheid B., Bausch-Fluck D., Henderson C., O’Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009). [2]Complementary expression of transmembrane ephrins and their receptors in the mouse spinal cord: a possible role in constraining the orientation of longitudinally projecting axons. Imondi R., Wideman C., Kaprielian Z. Development 127:1397-1410(2000). [3]The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). The MGC Project Team Genome Res. 14:2121-2127(2004).Additional information
Size | 50μg, 100μg, 50μl, 100μl |
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