Aliases
Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog, RAD17, R24L
Antibody Type
Polyclonal Antibody
Species
Human
Uniprot ID
O75943
Immunogen
Recombinant human Cell cycle checkpoint protein RAD17 protein (1-270AA)
Raised In
Rabbit
Species Reactivity
Human,Mouse
Tested Applications
ELISA, WB, IHC;Recommended dilution:WB:1:500-2000, IHC:1:20-1:200
Background / Function
Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Literature
[1]”Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.”Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. Poustka A.Genome Res. 11:422-435(2001). [2]”The full-ORF clone resource of the German cDNA consortium.” Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007). [3]”The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9.”Rauen M., Burtelow M.A., Dufault V.M., Karnitz L.M. J. Biol. Chem. 275:29767-29771(2000).
