Aliases
Rep, 40.4 kDa protein, Protein AC1, Protein AL1, AC1, AL1
Antibody Type
Polyclonal Antibody
Species
African cassava mosaic virus
Uniprot ID
P14982
Immunogen
Recombinant African cassava mosaic virus Replication-associated protein (1-358AA)
Raised In
Rabbit
Species Reactivity
African cassava mosaic virus
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5′-TAATATTAC-3′ in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5′-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3′-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities (By similarity).
Isotype
IgG
Conjugate
FITC
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Literature
[1]”Nucleotide sequence of cassava latent virus DNA.” Stanley J., Gay M.R. Nature 301:260-262(1982).
