Description
Aliases
AIRE1; APECED; APS1; APSI; PGA1
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: O43918;
NCBI Gene#: 326;
NC
Immunogen
Peptide sequence around phosphorylation site of Serine156 P-G-S(p)-Q-L) derived from Human AIRE.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB Recommended dilution: Western blotting: 1:500~1:1000
Background / Function
The function of the protein encoded by this gene is not well defined, however it contains zinc finger motifs suggestive of a transcription factor. The protein (isoform 1) is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described [1]. The longer AIRE-1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE-2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE-3 includes the same exon 1-8-9 sequences as found in AIRE-2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized. Nagamine K., Nat. Genet. 17:393-398(1997).Aaltonen J., Nat. Genet. 17:399-403(1997).Hattori M., Nature 405:311-319(2000).
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser156
Additional information
| Size | 100?l |
|---|
