Description
Aliases
Acute myeloid leukemia 1 protein; CBFA2; CBFA2; alpha 2 subunit; RUNX1
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: Q01196;
NCBI Gene#: 861;
NC
Immunogen
Peptide sequence around phosphorylation site of Serine 435( S-N-S(p)-P-T) derived from Human AML1.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB Recommended dilution: Western blotting: 1:500~1:1000
Background / Function
CBF binds to the core site, 5′-PYGPYGGT-3′, of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.Miyoshi H., Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434(1991).Sacchi N., Genes Chromosomes Cancer 11:226-236(1994).Nucifora G., Blood 81:2728-2734(1993).
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser435
Additional information
| Size | 100?l |
|---|
