Description
Source protein:
Protein A affinity
chromatography purified protein from mouse ascites fluid.
Clonality and Host Species:
E32, Mouse
Immunogen and Isotype:
Recombinant full length
ERK1 protein, IgG1
Specificity and reactivity:
Detects the ERK1 protein
(e.g. from human and mouse cells).
Application:
IP, Western blot
Available form:
Lyophilized 1mg/mL (lyophilized
in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN3)
Working procedure:
Western blot (with ECL)
1:1000-10,000 dilution incubates for 1 hour at room temperature. For the best
results, determine the optimal working antibody dilution for your protein of
interest.
Storage:
Supplied lyophilized
antibodies can be stored at -200C for 3 years.
Reconstitute the
lyophilized antibody with distilled water to 1mg/ml or a desired concentration
before use.
Reconstituted liquid can be
stored at -20oC for 2 years. Centrifuge after first thaw to maximum
product recovery. Aliquot into smaller volume to avoid repeated freeze-thaw
cycles. Store aliquots at 40C
for several days to 2 weeks.
Additional information
| Size | 50 µg, 100 µg |
|---|
