Anti-GAPDH, Clonility GAIR

General Description

Source protein:                              Protein A affinity purified protein from mouse ascites fluid

Clonality and Host Species:        GA1R, Mouse

Immunogen and Isotype:             Recombinant GAPDH, IgG1

Specificity and reactivity:            Detects native and denatured forms of GAPDH (~37 KDa) from bacteria, yeast, rat, mouse, rabbit, chicken, human and hamster

Application:                                    Western Blot, Dot Blot, ELISA, IHC/IS

Available form:                              50 µg of lyophilized antibody (lyophilized in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN₃)

Working procedure:                      Western blot (with ECL) 1:1000-100,000 dilution, incubate for 1 hour at room temperature. For the best results, determine the optimal working antibody dilution for your protein of interest.

Storage:                                           Supplied lyophilized antibodies can be stored at -20^ºC for 3 years. Reconstitute the lyophilized antibody with distilled water to 1mg/ml or a desired concentration before use. Reconstituted liquid can be stored at -20^ºC for 2 years. Centrifuge after first thaw to maximum product recovery. Aliquot into smaller volume to avoid repeated freeze-thaw cycles. Store aliquots at 4^ºC for several days to 2 weeks.